Synthesis and Purification of Peptide Nucleic Acids
نویسندگان
چکیده
منابع مشابه
Synthesis of Glycol Nucleic Acids Synthesis of Glycol Nucleic Acids
SYNTHESIS 2006, No. 4, pp 0645–0653xx.xx.2006 Advanced online publication: 25.01.2006 DOI: 10.1055/s-2006-926313; Art ID: M05505SS © Georg Thieme Verlag Stuttgart · New York Abstract: Starting from glycidol, the synthesis of dimethoxytritylated glycol nucleoside phosphoramidites of adenine (A), thymine (T), uracil (U), guanine (G), and cytosine (C) is reported. These phosphoramidites are the bu...
متن کاملFacile synthesis of peptide nucleic acids and peptide nucleic acid-peptide conjugates on an automated peptide synthesizer.
Peptide nucleic acids (PNAs) are DNA mimics with a neutral peptide backbone instead of the negatively charged sugar phosphates. PNAs exhibit several attractive features such as high chemical and thermal stability, resistance to enzymatic degradation, and stable binding to their RNA or DNA targets in a sequence-specific manner. Therefore, they are widely used in molecular diagnosis of antisense-...
متن کاملAntisense properties of peptide nucleic acids.
PNA is a nucleic acid analog with an achiral polyamide backbone consisting of N-(2-aminoethyl)glycine units (figure 1). The purine or pyrimidine bases are linked to the each unit via a methylene carbonyl linker (1-3) to target the complementary nucleic acid (4). PNA binds to complementary RNA or DNA in a parallel or antiparallel orientation following the Watson-Crick base-pairing rules (5-7). T...
متن کاملSynthesis and Application of Functional Nucleic Acids
1 Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20, Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan 2Physics Department, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany 3Proteomics and Structural Biology Unit, Institute of Genomics and Integrative Biology, CSIR, Mall Road, New Delhi 110 007, India 4Departmen...
متن کاملSynthesis of cell-permeable peptide nucleic acids and characterization of their hybridization and uptake properties.
Guanidine-based peptide nucleic acid (GPNA) monomers and oligomers containing all four natural (adenine (A), cytosine (C), guanine (G), and thymine (T)) and two unnatural (2-thiouracil (sU) and 2,6-diaminopurine (D)) nucleobases have been synthesized. Thermal denaturation study showed that GPNA oligomers containing alternate D-backbone configuration bind sequence-specifically to DNA and, when i...
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ژورنال
عنوان ژورنال: Current Protocols in Nucleic Acid Chemistry
سال: 2002
ISSN: 1934-9270,1934-9289
DOI: 10.1002/0471142700.nc0411s09